Electrical impedance myography: transitioning from human to animal studies.

OBJECTIVE To determine the feasibility of performing electrical impedance myography (EIM) in rats. METHODS EIM was performed on the hamstring muscles of 6 healthy adult rats with applied frequencies of 2-300 kHz. Studies were performed over a 6-week period, with 3 rats having recordings made from the skin (surface EIM) and 3 with recordings directly from the muscle (direct-muscle EIM). In addition, sciatic nerve crush was performed on one rat and comparisons made pre- and post-injury. Reactance and resistance were measured and the primary outcome variable, the phase angle (theta), calculated. RESULTS EIM patterns in the rat hamstring muscles were qualitatively similar to those observed in human subjects. This held true for both surface and direct-muscle recordings, although direct-muscle data appeared less repeatable. Sciatic nerve crush data in the single rat showed a dramatic reduction in phase and a relative loss of frequency-dependence. CONCLUSIONS EIM data similar to that obtained from human subjects can be acquired from rat muscles with surface recordings proving more consistent and easier to obtain than direct-muscle recordings. Changes seen with sciatic nerve crush mirror those seen in patients with neurogenic injury. SIGNIFICANCE These results support the possibility of performing EIM on rat models of neuromuscular disease.